Direct nonradioactive in situ hybridization of somatic cell hybrid DNA to human lymphocyte chromosomes
نویسندگان
چکیده
منابع مشابه
Direct fluorescence in situ hybridization to polytene chromosomes.
Fluorescence in situ hybridization (FISH) and its modification, polytene in situ hybridization (PISH) (1,2,4), have widespread application in the analysis of gene localization on interphase, metaphase and polytene chromosomes. However, available protocols use the indirect fluorescence approach, which requires the tedious and timeconsuming immunological detection step in which a fluorochrome-con...
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Spatial and temporal visualization of RNA transcripts in tissue is a key tool in studying both developmental and pathological processes. In situ hybridization is a highly sensitive method for RNA transcript detection. It is based on sequence complementation between a labeled RNA probe and the RNA transcript of interest. The labeled probe is then detected by immunohistochemical methods using an ...
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Combined with a system for identifying each of the chromosomes in a genome, visualizing the location of individual genetic loci by fluorescence in situ hybridization (FISH) would aid in assembling physical and genetic maps. Previously, large genomic clones have been successfully used as FISH probes onto somatic chromosomes but this approach is complicated in species with abundant repetitive ele...
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The differentiation of homologous chromosomes as well as their parental origin can presently be conducted and determined exclusively by molecular genetic methods using microsatellite or SNP analysis. Only in exceptional cases is a distinction on a single-cell level possible, e.g. due to variations within the heterochromatic regions of chromosomes 1, 9, 16 and Y or the p-arms of the acrocentric ...
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DNA in situ hybridization (DNA ISH) is a commonly used method for mapping sequences to specific chromosome regions. This approach is particularly effective at mapping highly repetitive sequences to heterochromatic regions, where computational approaches face prohibitive challenges. Here we describe a streamlined protocol for DNA ISH that circumvents formamide washes that are standard steps in o...
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ژورنال
عنوان ژورنال: Cytometry
سال: 1990
ISSN: 0196-4763,1097-0320
DOI: 10.1002/cyto.990110112